A simple tool to identify the biomass precursors which the microorganism is unable to synthesize upon deletion of essential, synthetic lethal (and higher order thereof) genes (reactions). This generic algorithm can be used to identify the constituent precursors to any sink metabolite the microorganism is unable to synthesize such as, biomass, lipid, cholesterol and so on.
Read the full description of the Precursor Identifier algorithm here.
Formulation:
Related Publications:
Chowdhury R., Chowdhury A., Maranas C.D. (2015), "Using gene essentiality and synthetic lethality information to correct yeast and CHO cell genome-scale models", Metabolites, 5(4):536-570. PMID: 26426067.
The code is freely available for academic users. Before downloading our software, we ask for your name and institution so we may better design future versions of our software. Every consideration has been made during the design, writing, and posting of this code to ensure the program works properly.