We have constructed a kinetic model of Escherichia coli core metabolism that satisfies the fluxomic data for wild-type and seven mutant strains. This model encompasses 138 reactions, 93 metabolites and 60 substrate-level regulatory interactions accounting for glycolysis/gluconeogenesis, pentose phosphate pathway, TCA cycle, major pyruvate metabolism, anaplerotic reactions and a number of reactions in other parts of the metabolism. We have developed an optimization-driven parameter identification method and integrated it with the Ensemble Modeling (EM) procedure for model parameterization.
Khodayari A., A.R. Zomorrodi, J.C. Liao and C.D. Maranas (2014), "A kinetic model of Escherichia coli core metabolism satisfying multiple sets of mutant flux data". Metabolic engineering, 25C:50-62.
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