Mycoplasma genitalium is one of the smallest known self-replicating organisms. We have developed a metabolic model for M. genitalium, iPS189, that contains 262 reactions and 272 metabolites. It incorporates 189 of the 482 predicted open reading frames in M. genitalium. This model is 87% accurate in recapitulating in vivo gene essentiality results for M. genitalium. Additionally, the model is 87% (i.e., 149 of 171 genes) correct in its essentiality predictions (specificity) and 89% (i.e., 16 of 18 genes) correct in its non-essentiality predictions (sensitivity).
Suthers, P.F., M.S. Dasika, V. Satish Kumar, G. Denisov, J.I. Glass and C.D. Maranas (2009), "A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189," PLoS Computational Biology, 5(2): e1000285.
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